BACE1 antibody

Catalog No. ABIN6774191

The Mouse Monoclonal anti-BACE1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect BACE1 in samples from Human.

Quick Overview for BACE1 antibody (ABIN6774191)

Target: BACE1 (Beta-secretase 1 (BACE1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BACE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-BACE1 Antibody

Specificity: Recognizes human BACE-1. This antibody was selected for its ability to detect human or mouse BACE-1 in Western Blots and direct ELISAs. In Western Blots, no cross-reactivity was observed with rhBACE-2, rmBACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE. Species cross-reactivity: Mouse.

Purification: Protein G purified

Immunogen: Synthetic peptide from human BACE1.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, WB (1:10000)

Usage: Western Blot-This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human or mouse BACE-1. Using a colorimetric detection system, the detection limit for rhBACE-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Flow Cytometry-Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA-This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human or mouse BACE-1 coated on 96-well plates. The detection limit for rhBACE-1 is approximately 1 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 1 mL of Sterile PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Aviod frequent freeze thaws.

Storage: -20 °C

Storage Comment: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Target Details for BACE1

Target: BACE1 (Beta-secretase 1 (BACE1))

Alternative Name: BACE1 / BACE

Background: Name/Gene ID: BACE1
Subfamily: Aspartic A1
Family: Protease

Synonyms: BACE1, APP beta-secretase, Asp 2, ASP2, Aspartyl protease 2, Aspartylprotease 2, Beta-secretase, Beta-site APP-cleaving enzyme, BACE, HSPC104, KIAA1149, B-Secretase, Beta-secretase 1, Memapsin-2

Gene ID: 23621

UniProt: P56817